PRODUCTION AND PURIFICATION OF BACTERIOCIN
FROM LACTOBACILLUS BREVIS
V. MUTHUMANI AND V. GUNASUNDARI
Abstract
This study was to determine the production of Bacteriocin from Lactobacillus brevis. The different
colonies were collected from cow milk and gram staining was performed for identification of gram positive
lactic acid bacteria. The test organisms were inoculated into lactobacillus agar medium for pure culture.
Lactobacillus brevis was selected test organism for the production of bacteriocin, confirmed by using gram
staining and biochemical tests, then the Lactobacillus brevis cells were taken in different time points such as
12 hrs, 24 hrs, 36 hrs, 48 hrs and 72 hrs to study the time kinetics of the strain of interest. The best growing
culture were centrifuged at 12000 rpm for 10 minutes at 4 oC. After 36 hrs, the Lactobacillus brevis culture
were subjected into Heat shock treatment at 55 oC, Cold shock treatment at 4 oC and pH variation of the
medium maintained at pH 9 for bacteriocin production. The produced bacteriocins were allowed to
antimicrobial assay.Then bacteriocin was partially purified by using Column chromatography technique.
Again SDS PAGE was performed to determine the molecular weight of the bacteriocin.
Enter your contact information below to receive full paper.
|
Journal Issues |
Vol 22, Issue 4, 2020
Vol 22, Issue 3, 2020
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
|
|