CALLUS INDUCTION AND PLANT REGENERATION IN SESAME
(SESAMUM INDICUM L.) - A LESSER EXPLOITED OIL CROP
WITH IMMENSE POTENTIALITY
DEEPA SINGH KUSHWAHA, SHAGUFTA KHAN AND ZIA-UL-HASAN
Abstract
An in vitro propagation protocol has been developed for Sesamum indicum L., cv GT-1.The
maximum seedling growth and seedling length was achieved in MS media. Better growth of seedlings
was, however, obtained on MS ½ strength medium and maximum seedling length was achieved in both
MS ½ and MS ¼ medium. Among different concentration of BAP, maximum seedling growth and
seedling length was obtained on its 0.1 mg/L concentration. Cotyledonary leaf segments was obtained
from 10 days old in vitro raised seedlings through in vitro seed germination. Highest callus induction
(80%) was achieved in 2.0 mg/l 2,4-D with 2.0 mg/L Kn. Ms medium containing 6.0 mg/L BAP with 2.0
mg/L NAA was found most suitable for shoot initiation. Best shoot regeneration (85%) were achieved
from 40 days old callus. Although shoot multiplication was achieved on MS medium containing BAP,
NAA and CW, the maximum number of shoots (20) and shoot length (5 cm) were obtained in 6.0 mg/L
BAP with 20% CW, 6.0 mg/L BAP with 0.5 mg/L NAA and 8.0 mg/L BAP. During multiplication maximum
callusing observed in 6.0 mg/L BAP with 20% CW. Highest rooted shoots (85-90 %) and number of roots
(8-10) were observed on MS medium containing 2.0 mg/L IBA. During acclimatization survival of
plantlets with up to 80 - 85% survival.
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