Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

CALLUS INDUCTION AND PLANT REGENERATION IN SESAME (SESAMUM INDICUM L.) - A LESSER EXPLOITED OIL CROP WITH IMMENSE POTENTIALITY

DEEPA SINGH KUSHWAHA, SHAGUFTA KHAN AND ZIA-UL-HASAN

Abstract

An in vitro propagation protocol has been developed for Sesamum indicum L., cv GT-1.The maximum seedling growth and seedling length was achieved in MS media. Better growth of seedlings was, however, obtained on MS ½ strength medium and maximum seedling length was achieved in both MS ½ and MS ¼ medium. Among different concentration of BAP, maximum seedling growth and seedling length was obtained on its 0.1 mg/L concentration. Cotyledonary leaf segments was obtained from 10 days old in vitro raised seedlings through in vitro seed germination. Highest callus induction (80%) was achieved in 2.0 mg/l 2,4-D with 2.0 mg/L Kn. Ms medium containing 6.0 mg/L BAP with 2.0 mg/L NAA was found most suitable for shoot initiation. Best shoot regeneration (85%) were achieved from 40 days old callus. Although shoot multiplication was achieved on MS medium containing BAP, NAA and CW, the maximum number of shoots (20) and shoot length (5 cm) were obtained in 6.0 mg/L BAP with 20% CW, 6.0 mg/L BAP with 0.5 mg/L NAA and 8.0 mg/L BAP. During multiplication maximum callusing observed in 6.0 mg/L BAP with 20% CW. Highest rooted shoots (85-90 %) and number of roots (8-10) were observed on MS medium containing 2.0 mg/L IBA. During acclimatization survival of plantlets with up to 80 - 85% survival.