ISOLATION AND CHARACTERIZATION OF PROANTHOCYANIDIN FROM VITIS VINIFERA BY CHROMATOGRAPHIC AND SPECTROSCOPIC TECHNIQUESSHANMUGA PRIYA AND R. DEVIKA
Vitis vinifera (Common grape) is common and native of Mediterranean region, Central Europe and Southwestern Asia. Currently, there are 5,000=10,000 varieties of species under the genus Vitis which have commercial and medicinal significance. V. vinifera contains many phenolic compounds (Anthocyanins, Hydroxycinnamic acid, Tannins) in the skin, pulp and seeds are rich of proanthocyanidins. Proanthocyanidin represent a group of condensed flavan-3-ols (Procyandins, predelphinidins, propetargonidins) and have significant therapeutic valve in the traditional medicine. In the present investigation, the seeds of Vitisvinifera were collected, dried, finely powdered and subjected for extraction using ethanol as a solvent. The ethanolic extraction was subjected to silica gel and sephadex gel permeation chromatography. About seven to eight fractions were identified and proanthocyanidin was found to be in the fraction 2 with retention time 2.97 as reflected by HPLC. For each fraction the molecular weight of the eluted compounds was determined directly by LSIMS (ranging from 200-700Da). Further the C and H NMR spectrum clearly confirmed all the characteristic band of proanthocyanidin resonates.
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