Ecology, Environment and Conservation Paper

Vol 21, Issue 1, 2015; Page No.(385-390)

BIOCHEMICAL EVALUATION FOR THE SUCKING PEST TOLERANCE AGAINST DIFFERENT BT COTTON HYBRIDS

S.V. Nehete, Swati S. Gurve, Ram K. Wargantiwar, Atul D. Gonde and P.K. Lokhande

Abstract

Biochemical studies were carried out against eight Bt cotton hybrids Bunny, Dhuv, Monsoon, NHH-44, Rasi-2, Dyna, Mallika and Tulasi at the MPKV Rahuri, revealed the total polyphenol content in leaves of Bt cotton hybrids at 30 DAS maximum of 6.05 mg g-1 fr.wt. being present in Dyna. At 60 DAS maximum of 7.91 mg g-1 fr.wt. being present in NHH-44 and 90 DAS maximum of 8.41 mg g-1 fr.wt. in Tulasi. The tannin content in leaves at 30 DAS maximum of 3.98 mg g-1 fr.wt. being present in Dhruv at 60 DAS maximum of 8.74 mg g-1 fr.wt. being present in Monsoon and at 90 DAS the maximum of 8.35mg g-1 fr.wt. being present in Monsoon.The chitinase activity in leaves of Bt cotton hybrids the maximum of 4.75 mg Nacetylglucosamine released g-1 fr.wt. hr -1 being present in Dyna at 60 DAS the maximum of 4.98 mg g-1 fr.wt. hr-1 being present in Rasi-2 and at 90 DAS the maximum of 6.65 mg g-1 fr.wt. hr-1 being present in Dhruv. The peroxidase activity the maximum of 2.24 units g-1 fr. wt. being present Dhruv at 60 DAS the maximum of 2.65 units g-1 fr. wt. being present in Dyna and at 90 DAS peroxidase activity ranged from 2.13 to 5.00 units g-1 fr. wt.. The maximum of 5.00 units g-1 fr. wt. being present in Monsoon and the minimum of 2.04 units g-1 fr. wt. in Tulasi. The polyphenol oxidase activity in leaves of eight Bt cotton hybrids ranged from 0.85 to 2.22 units g-1 fr. wt. at 30 DAS. The maximum of 2.22 units g-1 fr. wt. being present Dyna and the minimum of 0.85 units g-1 fr. wt. Monsoon and at 60 DAS ranged from 1.03 to 2.56 units g-1 fr. wt. The maximum of 2.56 units g-1 fr. wt. being present in Dyna and the minimum of 1.03 units g-1 fr. wt. in Monsoon. At 90 DAS ranged from 1.03 to 3.22 units g-1 fr. wt. The maximum of 3.22 units g-1 fr. wt. being present in Dyna and the minimum of 1.03 units g-1 fr. wt. in Dhruv is moderately resistant against the sucking pest infestation and higher biochemical constituents at different growth stage.

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