THE EFFECT OF ADDITIONAL CARBON AND NITROGEN SOURCES ON GA 3 BIOSYNTHESIS BY BACILLUS CEREUS ISOLATED FROM THE RHIZOSPHERE OF SUGARCANEN.D. PANDYA AND P.V. DESAI
In this study, favorable carbon-nitrogen ratio for high yields of gibberellic acid (GA3) production from Bacillus cereus was investigated. First of all, optimum carbon (glucose, sucrose, maltose) and nitrogen (NH4Cl, NaNO3, urea) sources among the others were chosen. The highest yield of GA3 productivity was found in growth medium supplemented with glucose (445.52±0.45 mg/L). NaNO3 was found as a suitable nitrogen source (424.18±0.18 mg/L). Then, in order to determine the optimum carbon-nitrogen ratio, different concentrations of carbon (from 50 mM to 200 mM) and nitrogen (from 10 mM to 40 mM) sources were added in culture media. As a result, optimum carbon- nitrogen ratio for GA3 production from Bacillus cereus was found to be 100:10 mM.
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