PARTIAL PURIFICATION, CHARACTERIZATION AND LAUNDRY APPLICATIONS OF PROTEASE FROM BACILLUS SP. KCTISARASWATHY, RAMALINGAM, SUJANA, JOY CHRISTINA AND RAJASEKARAN
Thermostable extracellular protease producing bacterial strain was isolated f b from SO1 y skim milk zone clearing activity. Protease from Bacillus spp. KCT1 was partially purified and characterized. The optimum temperature and pH for the protease was found to be 55°C and pH of 10.5, respectively. The metal ions like Ca2+, Me, and Al3+ enhanced enzyme activity. The protease activity was completely inhibited by phenylmethanesulfonyl fluoride (PMSF). Compatibility study with commercial detergents showed that the enzyme was able to retain its 50% of initial activity after 1.5 hours of incubation in the detergent solution. The enzyme was found to be effective for the removal of blood and egg yolk stains in cotton cloth.
Enter your contact information below to receive full paper.