Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 7 Issue 4, 2005; Page No.(899 -902 )

CALLUS INDUCTION AND CYTOKININ SPECIFIC ORGANOGENESIS IN MORFTLLS ALBA LEAF DISCS

R.S. CHUDASAIVIA, S.H. PANSURIYA, S.B. BAGATHARIA AND V.S. THAKEK

Abstract

Plant hormones such as auxins and cytokinins play a vital regulatory role in plant cell, tissue and organ development in vivo or I'm vitro. Leaf discs of white mulberry (MofSus allni) were subculture^ in Murashige and Skoog media containing various concentrations of KiN, BAP, 2,-1-D and NAA to study ilnir influence on callus induction and organogenesis. When leaf .discs were cultured on a medium containing different ratio of NAA:BAP i.e. 0.81,1.1 and 2.4 or NAA:KiN 1.2 only profound callus growth was observed, after three weeks of inoculation. Nearly 80 to 100% callus induction wns observed after three to four weeks in these concentrations. In the present study instead of BAP when KiN is used with NAA in .1 ratio ranging from 0.5 to 2.0 a gradual induction of root from the callus cells was observed and when NAA and KiN used in a ratio of_2j2, maximum root production was observed and percent callus induction remarkably. This results suggests that when same combination of NAA was used with different source of cytokinins i.e.KiN instead of BAP, rooting was observed.

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