CHARACTERIZATION OF A VIRUS ISOLATED FROM CHANNA GACHUA AFFECTED WITH EPIZOOTIC ULCERATIVE SYNDROMEALOK TRIPATHI, RAVINDRA CHOPADE AND T.A. QURESHI
This is the first report of the isolation and the characterization of virus from EUS affected fish from India. The virus was isolated from the skin, liver and kidney of Channa gachua in severely lesioned stage. The virus inoculated, induced cytopathic effects (CPE) in CGL/14 and CGK/14 yielding a titer of 3.02x106TCID50/mL at 25oC temperature within 2 to 3 days. Temperature ranging between 15 to 25oC was optimum for replication of virus but it did not replicate at 37oC. The virus could be stored at -10 and 8 oC for 30 and 10 days respectively without any significant loss of infectivity. A 1-log difference was noticed TCID50 titer between the cell-free virus and total virus. Freezing and thawing virus caused a half-log drop in titer. Viral exposure to chloroform or heating upto 56oC for 30 minutes inactivated the virus. Exposure to pH3 medium for 30 minutes resulted in more than 100-fold loss of viral infectivity. The iododeoxyuridine (lUdR) did not affect virus replication, indicating its RNA genome. Neutralization tests using the antisera of RE-19E virus and the ulcer disease rhabdovirus (UDRV) obtained from AAHRI, Thailand showed a cross- reaction. Cross-reaction between Indian virus antiserum and the antisera obtained from Thailand was positive and established serological relationship with SHRV. Transmission electron microscopy (TEM) of CGL/14 and CGK-14 cell lines showed virus particles with typical bullet shape morphology and measured approximately 65x175mm in size. Thus the virus isolated from Indian snakehead fish is characterized rhabdovirus.
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