Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 3, 2008; Page No.(601-605)

ISOLATION AND VREPARATION OF CARRIERS WITH PHOSPHATE SOLUBLIZING BACTERIA

V.JEYANTHI KUMARI AND B.VICTOR

Abstract

An attempt was made to analyse the presence of Phosphate Solublizing Bacteria (PSB) in a rhizosphere soil of paddy field, also their ability to survive in various carrier-based materials, such as compost soil, ash, activated charcoal powder and Gumarabic powder. Rh izosphere soil of Oniza sativa was examined for Total Heterotrophic Bacterial Population (THBP) and Phosphate Solublizing Rhizosphere Soil Bacterial Isolates (PSRSBI). Comparing the data of THBP and PSRSBI, they existed an inverse relationship between them. A total of 25 PSRSBI were characterized to its generic level and was principally composed of Pseudomonns sp; Bacillus sp and Micrococcus sp in the order of 48%, 44% and 8% respectively. The two PSRSBI namely Pseudomonas sp; Bacillus sp were selected and examined for their ability to survive and multiply in the above said carrier materials as mono and co-cultures. Results inferred that the compost soil supported the propagation of the strains more efficiently and were true especially when the mixed cultures were inoculated. The Pseudomonas sp mixed with compost soil carrier showed a marginal increase in it's survival when compared with Bacillus sp. The result of ash, when compared with compost soil, the mixed cultures propagated much strongly than the individual inoculums. The charcoal has rendered only marginal increase in the bacterial population. The Gumarabic powder has exhibited a lower level of incorporated organisms, but it was more pronounced on Pseudomonas sp compared to other inoculums.

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