Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 2, 2007; Page No.(249-254)

KINETICS OF EXTRA CELLULAR PROTEASE PRODUCTION BY MUTANT A. NIGER ABIOO DURING FISH SCALE SOLUBILISATION IN PRESENCE OF EXOGENOUS CARBON AND NITROGEN SOURCES

BARNALI BASU, MANAS DAS AND AJIT K BANIK

Abstract

Various attempts have been made to utilize waste material of food processing industries by hydrolysis with microbial protease for recovery of protein. Surface culture fermentation of fish scale was carried out with mutant Aspergillus niger A13100 to study the effect of exogenous carbon and nitrogen sources on kinetics of protease production as well as fish scale solubilisation. Glucose can exert a strong stimulatory effect on protease production through biomass growth and its autolysis. Glucose utilization was occurred at early phase of incubation with highest economic co-efficient at 10'h day in 88.8% biomass production and 55.6% of glucose utilization. Autolysis of cell begins after 10 days of incubation. Utilization of urea was occurred at later phase of fermentation. Protease production was reaches it's peak at 14th clay of fermentation and strongly correlated with higher amount of amino nitrogen formation as well as higher pH value of the fermentation medium.

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