BIOCEMENT PRODUCTION UTILIZING UREOLYTIC BACTERIA ISOLATES FROM CAVE PREMISES SOILS.S. MAGAR, P.S. KHADKE, G.A. KADAM1 AND S.A. KATEAbstract Biocement is a microbial induced calcite precipitation (MICP) process that occurs due to biochemical activities of Ureolytic bacteria. In present work, from caves premises soil sample, ten Ureolytic bacteria were isolated at 37 oC after 24h incubation on nutrients agar supplemented with 5% urea. Six ureolytic bacterial isolates (S2, S3, S4,W2, W3 and W4) has shown highest calcium carbonate precipitation range from 0.14g-1.2g in 25 ml medium after one week incubation,CaCO3 precipitation was confirmed by analytical test. Most efficient isolates (S2)was identified by 16SrRNA sequencing as Bacillus cereus BC1 and remaining isolates were identified as Bacillus tequilensis, Bacillus amyloliquificians, Paenibacillus thiaminolytics, Bacillus lincheniformis, and Bacillus subtilis, as per Bergeyâs manual of a systemic bacteriology and ABIS software. The isolate Bacillus cereus BC1, showed 173.30 U/ml urease activity by using standard ammonia graph. Biocement cube was significantly produced faster, time effective and Eco-friendly to overcome harmful impact of cement on environment by using these isolates. |