OPTIMIZATION STUDIES ON PRODUCTION OF BACTERIOCIN FROM BACILLUS ARYABHATTAIPathade A.G. and Bodhankar M.G.Abstract Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria and are active against other bacteria, either in the same species (Narrow spectrum) or across genera (broad spectrum). The optimization of medium and other conditions were studied with respect to carbon source, nitrogen source, pH, inoculum size, incubation period and incubation temperature using de Man Rogosa Sharpe (MRS) basal medium, and it was found that, Bacillus aryabhattai isolate showed maximum yields of bacteriocins in their respective optimized media as compared to basal medium. The medium and other conditions were optimized at 0.1-L flask level studies. The Bacillus aryabhattai is isolated from soil and identified by 16s rRNA gene sequencing and NCBI accession number is MG551546.1 and the phylogenetic tree is also prepared. The optimized conditions include 2% Glucose as carbon source, 1% NH4CL as nitrogen source, pH 6.0 in the MRS base medium, inoculum size of 6 % at 108 Colony Forming Units (CFU)/ml of the suspensions, incubation period of 48 h and 30 oC temperature of incubation. These optimized conditions were used in the further work. The antagonistic potential against common food bacterial pathogens like pathogenic Escherichia coli and Staphylococcus aureusis also carried out. |