BIOSYNTHESIS OF BACITRACIN IN SOLID-STATE FERMENTATION USING DIFFERENT SUBSTRATESShete P.B., Pharne A.G. and Pathade G.RAbstract A Bacillus isolate was obtained from soil sample. It was then studied for its cultural, morphological and biochemical characteristics. The isolate was tentatively identified as Bacillus subtilis. For solid-state fermentation of bacitracin, the isolate was grown on basal medium. Fermentation was carried out SSF using three different solid substrates, wheat bran, rice husk and defatted cotton seed oil cake. Bacitracin produced was detected by TLC with standard bacitracin. The product formed in wheat bran showed greater inhibitory activity against test organism, i.e. Micrococcus luteus with 20mm diameter of zone of inhibition. |