Ecology, Environment and Conservation Paper

Vol.30, Jan. Suppl. Issue, 2024; Page No.(S345-S347)

ANTIMICROBIAL ACTIVITY OF FENUGREEK (TRIGONELLA FOENUM GRAECUM) AND MARIGOLD (TAGETES ERECTUS) ON ISOLATED AMPICILLIN RESISTANT S. AUREUS

R.P. Raut, S.M. Gharat, A.M. Chauhan and M.A. Jha

Abstract

This study focuses on the isolation of ampicillin resistant S.aureus from soil collected from dumping site located in Boisar, Palghar, Maharashtra. Ampicillin resistant S.aureus was isolated on St. Salt mannitol agar containing 40 mg/ml of ampicillin. The MIC and MLC of the isolated culture was found to be 40 mg/ml and 50 mg/ml respectively. Characterization of isolated ampicillin resistant S.aureus was done by biochemical test. Antimicrobial activity of methanolic and aqueous extract of fenugreek and marigold leaves was studied on the isolated culture by agar cup and disc diffusion method respectively. Methanolic extract of fenugreek leaves, seeds and marigold leaves showed promising results.