Ecology, Environment and Conservation Paper

EFFECT OF CULTURING TIME, POSITION OF NODE ON EXPLANT ESTABLISHMENT AND SURFACE STERILIZATION PROCEDURE FOR IN VITRO MICROPROPAGATION OF GRAPE ROOTSTOCKS

Anupa, T., Somkuwar, R.G and A.K. Sharma and Alok Gupta

Abstract

The current study, titled “Studies on in vitro Surface sterilization and disinfection procedure for in vitro propagation of grape rootstocks was conducted at the Tissue Culture Laboratory, ICAR-National Research Centre for Grapes Pune, from 2018-19 and 2019-2020. maximum explant establishment (82.3 %) and (80.3 %) were recorded in Dogridge and 110 R, respectively. When compared to May, June and July, the month of ‘April’ is ideal for culturing nodal cuttings. Both rootstocks had a similar pattern in terms of the monthto- month establishment. Carbendazim @ 1 g/l for 2 hours was shown to be the most effective in reducing contamination and promoting plant establishment in Dogridge (11.6 %) and 110R (13.3) rootstocks respectively. In Dogridge and 110 R rootstocks, charcoal @ 1g/l was found to be effective in reducing browning (12.3 and 12.6 %) and contamination rate (11.3 and 11.0 %). In Dogridge and 110 R. Surface sterilization with mercuric chloride for 7-10 minutes was shown to be effective in reducing contamination rate for nodal explants in Dogridge (19.6 %) and 110 R (22.6 %).