Ecology, Environment and Conservation Paper

GC-MS ANALYSIS OF PHYTOCOMPONENTS IN AQUEOUS EXTRACT OF TULSI AND MULHETHI

Sachin Kumar, Harsh Dhankhar and Seema Kumari

Abstract

Extraction process specifically targets isolation of carbohydrates, glycosides, and secondary metabolites from leaves. These compounds play crucial roles in Mulethi and Tulsi’s biological activities, including antioxidant, antimicrobial, and anti-inflammatory properties. By extracting and analysing these components, a deeper understanding of these plants’ medicinal potential can be gained. The identification of the extracted compounds was done using various chromatographic techniques. Chromatography is a powerful tool that separates and characterizes complex mixtures. Techniques such as gas chromatography (GC) were employed to separate the individual components of the extract and determine their chemical structures. The aqueous extract was screened and qualitatively analysed to detect specific compounds. The analytical technique combines GC separation capabilities with MS detection and identification capabilities. It provides valuable information about the chemical composition of the extracts, allowing the identification of individual compounds present in the samples. These compounds are known for their pharmacological activities and are often responsible for plants’ medicinal properties.