THE USE OF BACTERIAL LACCASE IMMOBILIZED ON AGROWASTE AS A POTENTIAL ECOFRIENDLY TECHNIQUE OF AZO DYE DE-COLORIZATIONS.L. ODEDARA AND D.N. SHERATHIAAbstract In the modern world, the effluent of the textile industry contains azo dyes, which are considered an alarming threat to the environment. Crystal violet, malachite green, reactive red are the most common dyes which are wieldy used in textile industries. Due to the sophisticated structure of these dyes, they are mutagenic and carcinogenic to both humans and aquatic life. A viable alternative is an affordable and eco-friendly bioremediation technique using bacteria or their enzyme that can degrade the azo dyes. Azo dye contaminated samples were collected from the industrial area of Ahmedabad and Jetpur (Gujarat, India). Forty three isolates with various morphologies were employed in the dye de-colorization experiment. Among them four most promising isolates (E11, S32, S12 and J24) were selected which de-colorize the all three dyes. Agowaste materials with MBSS medium were used to produce laccase enzymes from S12. Laccase was partially purified with the help of pre-chilled acetone. Partially purified laccase was immobilized in sodium alginate beads with rice husks and check the de-colorization efficiency of our experimental dyes. From the results, immobilized laccase can degrade 55.29% crystal violet and 55.10% malachite green dye in 4hr of incubation. Because of its incredible de-colorization ability, immobilized laccase has quite the potential as a cost-effective, biologically beneficial strategy for treating the textile effluents. |