Ecology, Environment and Conservation Paper

REVERSE BREEDING-VERSATILE TOOL FOR CREATING HOMOZYGOUS PARENTAL LINES

Sateesh Reddy Kaluvai, Deshraj Gurjar and Vijay Sharma

Abstract

Reverse breeding is an innovative and highly desirable plant breeding technique that aims to create homozygous parental lines directly from heterozygous plants. This method involves manipulating meiosis to minimize genetic recombination in the chosen heterozygote, thereby removing meiotic crossing over. By utilizing this approach, spores gathered from either male or female plants will possess unaltered parental chromosomes, which have not undergone any recombination. Consequently, these spores can be grown in a laboratory environment to generate homozygous doubled haploid plants (DHs). The heterozygote can be perpetually reconstituted by selecting complementary parents from these DHs. Compared to conventional plant breeding, which struggles with stabilizing unknown heterozygous genotypes, reverse breeding can revolutionize future plant breeding. In addition, reverse breeding has various applications, such as chromosome-specific breeding, that could be explored further. This approach creates offspring using only one distinct chromosome of the parent plants instead of all the chromosomes