CHARACTERIZATION OF ANTIOXIDANT QUERCETIN EXTRACTED FROM ONION SKINS AND RADISH LEAVES USING MICROWAVE AND CONVENTIONAL EXTRACTION METHODSA. Parmar, Genitha I., A.K. Singh and Y. VermaAbstract This research focused on characterization, identification of previously extracted Quercetin from two identified vegetable waste (Onion skin, Radish leaves). The Free Radical Scavenging activity with respect to Ascorbic acid was examined. Extraction was done by three methods i.e. Microwave, Soxhlet and Maceration, after that purification was done with the help of column chromatography and quantification of Quercetin performed by high performance liquid chromatography. Structural characterization of purified Quercetin was carried out in FTIR spectroscopy and DPPH method was used for determination of antioxidant activity of Quercetin. Yield of Quercetin (14.44μg/g) and antioxidant activity (87.58%) was found maximum for Onion skin extract with 90% Ethanol (EtOH) by using microwave at 400W as compared to Radish leaves. FTIR confirms the purity and structure of isolated Quercetin. Onion skin showed higher amount of Quercetin with microwave extraction which has good antioxidant activity. |