A STUDY OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) IN PATIENTS PRESENTING WITH ANAEMIA AT A TERTIARY CARE HOSPITALAVANI DANGAR, PALAK PATEL, MIRAT DONGA, KRISHNA KANT SHIROMANI AND KIRAN DELWADIAAbstract
The most prevalent type of genetically inherited red blood cell disorder worldwide are hemoglobinopathies. Screening and accurate identification of hemoglobin (Hb) variants have become increasingly important in antenatal diagnosis and prevention of Hb disorders. The objective is to identify haemoglobin abnormalities by high performance liquid chromatography (HPLC) for screening the anaemic patients who are visiting the hospital in OPD and admitted in IPD. This is cross sectional retrospective study for a period of 6 months and performed in a tertiary care hospital on patients presenting with anemia in different Departments. In total, high performance liquid chromatography HPLC of 400 patients with anemia was studied. Out of 400 patients, 190 were males and 210 were females. Age group varied from infants to middle-aged adults. The following criteria were used to identify hemoglobinopathies on HPLC patterns as follow: HbS:- 10%-45%: Sickle cell trait, 45%-50%: HbS>HbA-Sickle cell disease, HbS |