ANTIMICROBIAL POTENTIAL OF FUNGAL LACCASE ISOLATED FROM LIGNOCELLULOLYTIC WASTE SOILB. Nayak and R. ChoudharyAbstract The present study aims to investigate the antimicrobial properties of lignocellulytic fungal laccase. Antimicrobial enzymes target the different bacterial biofilms and their cellular components which can be used for bacterial control in healthcare, food manufacturing and environmental protection. Lignocellulytic fungi were isolated from lignocellulolytic waste soil sample (coconut waste soil, paddy straw waste soil, banana waste soil, citrus peel waste soil and flower waste soil) collected from Bhilai-Durg region of Chhattisgarh India. A total of 85 fungal strains were isolated by soil dilution technique on the fungal isolation medium i.e., Potato Dextrose Agar medium and Czapek Dox Agar medium. Primary screening was done by guaiacol plate assay method where guaiacol was used as indicator substrate. Only 17 fungal culture showed positive result for laccase production. Further all the laccase positive culture used for antimicrobial potential using the Kirby Baur well diffusion method against four Gram positive bacteria (Staphylococcus aureus, Myccrococcus leutis, Listeria monocytogens and Bacillus cereus) and six Gram negative bacteria (Salmonella enterica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeurogenosa, Aeromonas hydrophilia, and Enterococcus faecalis) were it showed better results against Gram positive bacteria than the Gram negative bacteria. |