DIGESTIVE ENZYMES (AMYLASE AND INVERTASE) ANALYSIS AND HISTOPATHOLOGICAL CHANGES IN OREOCHROMIS MOSSAMBICUS TREATED WITH TRICYCLAZOLE (75%) FUNGICIDEM. NoornissabegumAbstract Despite being efficient against rice blast fungal diseases when applied to agricultural fields, tricyclazole 75% fungicide has a variety of drawbacks that outweigh its benefits for the aquatic environment. Shortterm exposure to a sublethal dose of tricyclazole, fish O. mossambicus displayed altered digestive enzymes (amylase and invertase) as well as histological abnormalities in tissue organs like the skin, muscle, gills, liver, intestine, and brain tissues. The activity of amylase and invertase was found to decreasing and increasing in various concentrations. Histopathological changes on exposure to tricyclazole fungicides was found to increase in its pathological lesions of the tissue organs studied skin, muscle, gills, liver, intestine, and brain tissues of O. mossambicus noticed with necrosis, vacuolar degeneration, fusion and atrophy of the primary and secondary gill lamellae, degeneration of the cytoplasm in hepatocytes, atrophy, formation of vacuoles, rupture in blood vessels, damage in the dermis, concentrated melanophores, damaged blood vessels, and enlarged muscle fibers with increasing concentrations. Enzymatic alterations and pathological lesions in the tissue of O. mossambicus exposed to a toxicant at a sub-lethal concentration are a functional response of organisms that offers information on the nature of the toxicant. Hence, in the present study, in order to evaluate the environmental/aquatic pollution under experimental settings, an evaluation of the fishâs histological alterations and enzyme levels was carried out. In conclusion, fish are among the most susceptible aquatic species to harmful compounds; buildup of the fungicide in the fish may cause pathological problems that spread to people through the food chain. |