Ecology, Environment and Conservation Paper

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSESSMENT OF BERBERINE IN METHANOL EXTRACT OF DIVERSE PARTS OF TINOSPORA CORDIFOLIA

B.C. Akhilraj, J. Suresh, K. Rajamani, M. Kumar and R. Gnanam

Abstract

In the current work, a straight forward, affordable, reliable, repeatable, selective, and accurate high performance liquid chromatography (HPLC) approach for berberine measurement in both a 60% methanolic extract of Tinospora cordifolia and a commercial formulation was developed and validated. The berberine peak, which has a retention time of 9.52±1.03 min, is crisp and well-defined in the acetonitrile-water (25:75%, v/v) mobile phase. Berberine is estimated using HPLC at a wavelength of 266 nm and a flow rate of 1.0 ml/min. The calibration curve’s results from linear regression analysis demonstrates a strong linear association with a correlation coefficient of 0.997 in the concentration range of 0.5 to 50 g/ml. y=29716x- 4417.4 is the equation for the linear regression. The thresholds for quantification and detection are 0.18 and 0.55 g/ml, respectively. According to ICH criteria, the proposed technique has been verified for accuracy, precision, repeatability, and robustness. For the purpose of estimating berberine in methanolic extract and formulation, the proposed approach is used, which has a high degree of precision and accuracy. The approach is exact, repeatable, selective, and accurate for estimating berberine, according to statistical analysis.