EVALUTION OF JEEVAMRUTHAM (ORGANIC BIOFERTILIZER) FOR IT’S USE IN THE DEVISING AN NUTRIENT MEDIUM FOR THE ISOLATION OF BACTERIAShilpa S. Ruikar, Avadhoot Gharge and G.R. PathadeAbstract Jeevamrutham is widely used Organic biofertilizer prepared with cow waste, gram flour and Jaggery by fermentation process. The study was aimed at physicochemical and microbiological analysis of jeevamrutham and also to evaluate itâs use as a nutrient medium for isolation of bacteria. The Jeevamrutham prepared and subjected to physicochemical, and microbiological analysis. The filtrate of the Jeevamrutham was studied for its use as a Nutrient (Jeevamrut) medium for the growth of the bacteria. Total solid content of Jeevamrutham was found to be 100 mg/ml. The Standard Plate count (SPC) for Nitrogen fixers, for Phosphate solubilizing bacteria was found to be maximum on 6th day of fermentation. The SPC for Yeast and Molds was found to be maximum on 8th day. The study regarding the evaluation of Jeevamrut medium showed excellent growth of test Gram positive and Gram negative bacteria on Jeevamrut medium. The pigment producers showed same pigmentation pattern on Jeevamrut agar and on conventially used Nutrient agar. The cost of the Jeevamrut medium was calculated per 100 ml of medium and was found to be 60.27% less than that of commercially available media. The Jeevamrut medium is economical, all the ingredients are easily available, cheap and ecofriendly. The Jeevamrut medium can be a best option to use as a Nutrient medium for the cultivation of bacteria in the laboratory. |