ISOLATION, SCREENING AND CHARACTERIZATION OF GELATINASEAND PROTEASE PRODUCING MICROORGANISMS FROMORGANIC KITCHEN WASTESnehal A. Masurkar and G.R. PathadeAbstract The present study was aimed at isolation, screening and identification of gelatinase andprotease producingbacteria from the kitchen waste. The study was also focused on the potential of isolates to produce enzymeprotease and gelatinase. The total of 157 bacterial isolates were obtained from the organic kitchen wastecollected from various nearby sources. The primary screening was done to screen the potentialof theseisolates for their enzymatic potentials on selective media. The 57 promising bacterial isolates were found to have enzymatic potentials for both gelatinase and protease. The screened isolates were subjected to secondary screening, amongst which five isolates showed maximumproduction of both gelatinase and protease. These five isolates viz. KW91, KW104, KW121,KW128, KW98 were the most promising isolates showing high degree of milk and gelatin hydrolysis which may be potent in faster degradation of domestic kitchen waste. |