L-ASPARAGINASE – A PROSPECTIVE ANTICANCER ENZYME ONMCF-7 CELL LINES EXTRACTED FROM SOIL MICROFLORAA. Subhashini and G. Sangeetha VaniAbstract This study aims at investigating the soil bacteria for L asparaginase enzyme production and itsanti-cancereffect on MCF 7 cell line. In this study, 30 soil samples were screened for bacterial producing L asparaginaseenzyme. The soil samples were collected from various places in Ethiraj College, Chennai. Isolation wasdone using spread plate technique, biochemical and microscopic characterization for thepotential isolatesrevealed the bacterial genera as Bacillus, Escherichia and Pseudomonas species. Of the thirty samples collected,six isolates produced pronounced L asparaginase enzyme. These samples were further subjected to crudeenzyme extraction and estimation. The isolates S2 and Ni5 showed maximum production of enzyme with1090 units/ml of activity. Dialysis was carried out to concentrate the enzyme and molecular characterizationwas performed using SDS PAGE to separate the proteins according to the molecular weightwhich wasfound to be approximately 100 to 116 kDa. Anti-cancer activity was evaluated on MCF 7tumour cell line.The cell viability was dose dependent and IC50 of S2 was 62.5 μg and Ni5 31.2 μgrespectively. |