HYBRID PURITY ANALYSIS OF RICE USING MICRO SATELLITE MARKERSD. SHASHIBHUSHAN, T. PRADEEP AND RAVI KIRAN REDDY K.Abstract Rice is the staple food crop which can fight against hunger and poverty in most of the developing nations. Hybrids perform better than the traditional verities in both yield and quality aspects. But the main problem lies in the genetic purity of the hybrids. In this study, molecular test was conducted and compared with the grow out test to assess the genetic purity of the rice hybrid, JGLH1 at Seed Research and Technology Centre, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Hyderabad. JGLH1 and its parents were used as materials, using SSR molecular marker technology, to select primers and identify genetic purity according to its polymorphism. The SSR marker, XA 21 was amplified on the DNA extracted from the rice hybrid, JGLH1, and its parents, CMS 64A and JBR7. The amplification of marker was conducted on the DNA of 20 hybrid leaves and 1 leaf from each parent, separately. The marker was amplified at 650 bp in the parent, CMS-64A, and 730bp in JBR7, and amplified at both the base pairs in their F1 progeny, JGLH1. This confirms the hybridity of the rice hybrid JGLH1. The marker was unveiled in all the 20 leaves of the hybrid rice sample ensuring the purity of the seed lot. The grow out test results also showed 100% purity without any deviations. It concludes that molecular marker, XA 21 is effective in hybrid purity evaluation of the rice hybrid, JGLH 1. |