EVALUATION OF DEGRADATION POTENTIAL OF FREE CELL ANDIMMOBILIZED CELL USING SHAKE FLASK TECHNIQUE AND LABSCALE BIOREACTOR TECHNIQUE FOR REMEDIATION OFNITROAROMATICS IN AQUEOUS PHASEMeenu SharmaAbstract In this study, Free cell and Immobilized cell using Shake flask technique and lab scale Bioreactor techniquewas evaluated for the remediation of TNT in aqueous phase. In shake flask study, all samples with Freeand Immobilized cell culture were kept in a shaker for 24 hours at 120 rpm at room temperature whereasthe immobilized cell bioreactor was set at airflow 1 ml/l, agitation 50-60 rpm for 8 hours at 25 °C. Thequantitative analysis by HPLC in shake flask study revealed 82.37% and 98.12% of TNT degradation byFree cell culture and immobilized cell culture respectively after 24 hours and 81.19% of TNT degradationwas found in Bioreactor. The results depicted that Immobilized cell culture had better performance thanfree cell culture and immobilized cell bioreactor proved more effective than shake flask culture technique. |