PRODUCTION AND CHARACTERIZATION OF BIOEMULSIFIERFROM PROBIOTIC LACTOBACILLUS PLANTARUMM. ESTHER MAGDALENE SHARON, P. GEETHA AND A. ALFIND PAUL FRITAbstract Bioemulsifier production from a Probiotic Lactobacillus plantarum isolated from a rice basedfermented product was studied. The optimization of production of bio emulsifier as a function offermentation time in shaker and stationary conditions was carried out. The highest production of bioemulsifier with 38.63% decline in surface tension at 72 hoccurred under stationary conditions. The isolatedbio emulsifier is partially purified for characterization and anlaysis of CMC (0.45 g l1), oil displacement area,emulsification index, emulsifying activity and emulsion stability. Results showed that the bio emulsifier hadgood emulsifying property and FTIR analysis indicates the presence of proteins and polysaccharidesfractions. Aqueous solutions of the bioemulsifier displayed antimicrobial activity and antiadhesive activityagainst the pathogens: Escherichia coli ATCC, Escherichia coli ETCC, Yersinia enterocolitica and Staphylococcusaureus. All the results indicate that the bioemulsifier produced from L. plantarum can be utilized in foodformulation as a multiuse food additive. |