EXPLORING INDIGENOUS BACTERIAL ISOLATE FROM DYE EFFLUENT SITES FOR DECOLORIZATION OF BLUE HERD DYE SHETEU.R., Bhuktar J.J. and Jadhav A.G.Abstract Dye contaminated sites are the efficient source for isolating bacteria capable of dye decolorization and degradation. The effluent and soil samples were collected from industrial zone where such effluents are discharged. Collected samples were enriched in nutrient media containing 100 mg/l Blue Herd dye. Isolate was selected based on zone of clearance on soild media containing dye. Based on biochemical and 16S rDNA comparisons the isolate is identified as Bacillus subtilis UAS1. The isolate showed maximum decolorization at 37 oC temperature, pH 7.0, capable of removing 92% dye at 20 mg/l concentration and significantly remove at higher subsequently added dye concentration up to 200 mg/l. The decolorization was highest in presence of Sodium Molybdate, less with Tungastate, Silver Chloride, Zinc Sulphate, whereas no decolorization in presence of Copper sulphate. The strain B. Subtilis UAS1 also exhibits significant reduction in the COD values; this suggests its potential and significance in dye removal. |