Ecology, Environment and Conservation Paper

EFFECT OF MORINGA OLEIFERA LEAF EXTRACT ON THE HISTOPATHOLOGICAL FEATURES OF TESTICULAR SEMINIFEROUS TUBULES OF MICE (MUS MUSCULUS) EXPOSED TO METHYL MERCURY

Annise Proboningrat, Hani Plumeriastuti, Suzanita Utama, Sri Agus Sudjarwo, Djoko Legowo, Epy Muhammad Luqman and Widjiati

Abstract

This study aims to prove the effect of Moringa oleifera leaf extract on the histopathological features of seminiferous tubules of mice (Mus musculus) exposed to methylmercury. A total of 20 male mice were divided into five groups (C-, C+, T1, T2, T3) and treated for 21 days. C- given 0.01 ml of aquadest, C+ was given 0.4 mg/kg of methyl mercury, T1 was given 200 mg/Kg of Moringa oleifera leaf extract + 0.4 mg/Kg of methyl mercury, T2 was given 400 mg/Kg of Moringa oleifera leaf extract + 0.4 mg/Kg bw of methylmercury, and T3 were given 800 mg/Kg of Moringa oleifera leaf extract + 0.4 mg/Kg of methylmercury. The histopathological features of the seminiferous tubules were examined using the Modified Johnsen Scoring method. The data obtained were analyzed using the Kruskal-Wallis and Mann-Whitney tests. The results showed that Moringa oleifera leaf extract reduced the level of damage to the testicular Seminiferous tubules of mice (Mus musculus) exposed to methylmercury. Moringa oleifera leaf extract at a dose of 400 mg/ Kg bw gave the best protective effect compared to the doses of 200 and 800 mg/Kg bw.