THE UTILIZATION OF FRUIT WASTE EXTRACT IN PASAR INDUK OSOWILANGUN SURABAYA (PIOS) INDONESIA AS A RAW MATERIAL OF ETHANOL BIOCONVERSION BY SACCHAROMYCES CEREVISIAENITA CITRASARI, IRMA ARDIANA PUSPITA AND AGUS SUPRIYANTOAbstract This study was aimed to determine the effect of the variations of extract concentration, the variation of the length of fermentation time, and the combination of both on ethanol produced. Ethanol fermentation used Pasar Induk Osowilangun Surabaya (PIOS) fruit wasteâs substrates that was extracted then added with 10% Saccharomyces cerevisiae. This study used 4x4 factorial design, 2 factors, and 3 replications. The first factor was the concentration of fruit wasteâs extracts (25, 50, 75 and 100%). Second factor was the length of fermentation time (3rd, 6th, 9th, and 12th days). Reducing sugar measurement used Luff Method and ethanol amount measurement used Pycnometri Method. Etanol amount was subjected to variance analysis with Two Ways Anova test (á 5%), continued with Duncan test. The treatment of fruit wasteâs extract concentration effected ethanolâs amount, the maximum concentration in 100 % fruit wasteâs extract (6.9%). The treatment of fermentation time effected ethanolâs amount, the optimum of fermentation time on the 9th days (5.15%). The combination treatment between the variations of fruit wasteâs extract concentration and fermentation time effected ethanolâs amount, the maximum 100% fruit wasteâs extract within the optimum 9th days fermentation (8.12%). |