POTENTIAL OF VARIOUS SELECTED SPECIES OF PSEUDOMONAS TO DECOMPOSE PHENOLAYSSAR NAHLÉ AND ISMAIL SAADOUNAbstract Potential of various selected species of Pseudomonas to decompose phenol as a sole carbon source has been investigated. Biochemical and culture morphology examination of the six different types of bacterial colonies recovered on the agar surface revealed that they mainly belonged to genus Pseudomonas. Growth of the tested bacterial isolates on phenol was determined by optical density (O.D.) readings at 600 nm, and their potential to decompose phenol was assessed by UV absorbance at 273 nm after incubating the tested Pseudomonas spp. in mineral salts medium (MSM) supplemented with 0.2% (w/v) of phenol as a sole carbon source for 12 days at 28 °C. Results indicated the removal of 648 mg/L of phenol by Pseudomonas putrefaciense (strain B41) as compared to 103, 102, 60, 29, and 13 mg/L for Pseudomonas sp. 1, P. cepacia, Pseudomonas sp. 2, P. acidovoranse and P. fluorescense, respectively. Pseudomonas putrefaciense (strain B41) appeared to be the most potent phenol degrading isolate and seems to play a significant role in the decomposition of toxic compounds such as phenol.
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