CHARACTERISTICS OF NATIVE ENTOMOPATHOGENIC BACILLUS SP. BK5.2 AS AN ENVIRONMENTAL FRIENDLY POTENTIAL AGENT FOR DISEASE VECTORS AND PLANT DISEASES CONTROLSalamun, Ni’matuzahroh, Fatimah, Izdihar Tsana, Nadiah Al-Batati and Imro Atul Kasanah
Biological control is one way to overcome the problem of disease vectors and plant diseases. Biological control can be carried out by utilizing living organisms, for example, group of Bacillus bacteria. This study aimed to determine the name of species of the native entomopathogenic Bacillus sp. BK5.2 isolated from natural breeding sites of Ae. aegypti in Baluran National Park East Java Indonesia, genetic similarity with other species of Bacillus, detect a toxin encoding gene, and hemolytic activity test. The species name was determined by DNA isolation of the genome Bacillus sp. BK5.2 and visualized by electrophoresis. Amplification of 16S rRNA gene was done by the Polymerase Chain Reaction (PCR) method. The resulting nucleotide sequences were analyzed for genetic similarity with another Bacillus. Detection of toxin encoding gene was done through PCR method visualized by electrophoresis. The hemolytic activity test was done to analyze its biolarvacidal activity. The results showed that Bacillus sp. BK5.2 has 99% genetic similarity with Bacillus thuringiensis strain B16. The results of the detection of toxin encoding gene was a Cry11 coding gene. The Hemolytic activity test on blood agar plate showed that positive results. B. thuringiensis (BK5.2) can be developed as an environmental friendly biological control agent against disease vectors and plant diseases.