Ecology, Environment and Conservation Paper

CHARACTERIZATION OF AEROMONAS HYDROPHILA BACTERIA ON DUMBO CATFISH (CLARIAS GARIEPINUS) FROM BUNGO JAMBI PROVINCE, INDONESIA

A. Indrawati, T. Wulandari, F.H. Pasaribu and A.B. Rifai

Abstract

This study aims to isolate, characterize and detect virulent genes of A. hydrophila bacteria that cause MAS disease in African catfish in the Bungo regency of Jambi Province on a molecular basis. The results of the study are expected to provide information on the microorganisms that contains in Aeromonas hydrophila cause MAS in catfish with a biofloc system in Bungo. The samples of research was 35 catfishs that had symptoms of MAS in a biofloc from Bungo. The sample used is in the form of liver, kidney, spleen and intestine swabs, and identification based on the Indonesian National Standard (SNI 7303.1-2015). Gene detection use PCR method to targeting the 16S rRNA gene and the aerA gene. aerA gene is an aerolysin virulence gene that causes Motile Aeromonas Septicemia (MAS). Based on the results of biochemical testing A. microscopic Aeromonas hydrophila is a bacteria Gram negative with a short rod shape, and confirm with specific media show 17 positive Aeromonas hydrophila isolates. The results of the detection of 16S rRNA genes of Aeromonas hydrophila from 17 tested show that 6 isolates are positive for 16S rRNA genes at 1500 bp. Detection of the aerA gene show 6 isolates of Aeromonas hydrophila have the virulent factor aerolysin at 309 bp.