ANTIFUGAL POTENCY AGAINTS CANDIDA ALBICANS (ATCC 10231) AND ITS ACTIVITY AS BIOSURFACTANT OF WNA 4.1.13 FERMENTED GROWTH OF SEDIMENT FROM MANGROVE WONOREJO SURABAYA INDONESIAC. Rahayuningsih, S. Chusniati, D. Handijatno, L. Maslachah, S. Sarudji and Rahmi Sugihartuti
The purpose of this study was to identify the genus of bacteria that produces biosurfactants and its potency as antifungal to Candida albicans (ATCC 10231). WNA bacteria 4.1.13 were isolated from Wonorejo mangrove sediments in Surabaya. The bacteria were identified by examining the morphology and and cell colony. Cell morphology identification was carried out by Gram staining and spores identification. Biosurfactant activity was carried out by oil spreading test, drop collapse test and parafilm test. Antifungal activity was performed by agar diffusion method. The results showed that WNA 4.1.13 isolates from Surabaya Wonorejo mangrove sediments were rod shaped, including Gram positive and had endospores located at the center of bacterial cells. Based on the morphological character of the colony and genus cell bacterial isolates WNA 4.1.13 including Bacillus sp. The result showed that in the broth collapse test, the droplets were flat, forming a clean zone in the oil spreading test and widened diameter in the parafilm test. Antifungal activity test showed inhibition zones formed by fermentation broth isolates of WNA 4.1.13. In conclusion, the fermentation of broth of WNA 4.1.13 isolates from the Wonorejo Surabaya have biosurfactant activity and antifungal potency against Candida albicans (ATCC 10231).