Ecology, Environment and Conservation Paper

Vol 26, Aug Suppl. Issue, 2020; Page No.(9-17)

THE RELATIONSHIP BETWEEN GENE EXPRESSION AND BIODEGRADATION OF CLORSPAN AND GROUND UP PESTICIDES BY PSEUDOMONAS AERUGINOSA BACTERIA

Beadaa Abdalqader Mahdii and Ahmed Mohammed Turke

Abstract

18 bacterial isolates were isolated from 23 soil samples polluted with different pesticides, from Baghdad districts on both sides of Karkh and Rusafa. Where two PCR and qPCR tests performed, the results of the qPCR test for the 18 bacterial isolates of P. aeruginosa bacteria showed that the highest recorded gene frequency value in the biological treatment of Clorspan (Chlorpyrifos) recorded by bacterial isolation (5) where it recorded (356666382.4), and the highest value of genetic frequency in the biological treatment of Ground up (glyphosate) pesticide recorded by bacterial isolation (3) and reached to (52498.9155). As for the results of the PCR test showed genetic differences in the bacterial strains among P. aeruginosa isolation, and that the highest similarity rate recorded by the isolates (8,3), it reached to 89%.