Ecology, Environment and Conservation Paper

THE NUMBERS OF LYMPHOCYTES AND CAPILLARIES IN SILICA EXPOSED LUNG TISSUES OF BALB/C MICE (MUSMUSCULUS)TREATED WITH MORINGAOLEIFERA LEAVES EXTRACT

Kusmiyati, Soedjajadi Keman, Muhammad Amin, Suwarno and Agoes Soegianto

Abstract

This study was conducted to describe the effect of M. oleifera leaves extract (MLE) on lymphocytes and capillaries numbers in silica-exposed lung tissues of Balb/c mice (Musmusculus). This was an experimental study with randomized post test-only control group design using 30 male Balb/c strain mice (Musmusculus),8-10 weeks, weighing 20-30g, which were divided into five groups. The groups were negative control,positive control, treatment group with silica exposure and treated with MLE 2 mg/20g BW, 5 mg/20g BW,and 8 mg/20g BW. Positive control and treatment group were treated with suspension of silica particles intratracheally at concentration 2.5 mg which were dissolved in 60 Micro L saline solution. After 90 days, mice were sacrificed. The variables were MLE, silica exposure, lymphocytes and capillaries number. Results showed that the treatment groups showed lower numbers of lymphocytes and higher numbers of capillaries than the positive control group. The numbers of capillaries in lung tissues of mice treated with MLE at all doses were higher than those of positive control group. Meanwhile, the numbers of lymphocytes in treatment groups treated with MLE at all doses were lower than those of positive control group. However, there was no statistically significant difference (One Way ANOVA, p>0.05). In conclusion, administration of MLE potentially decrease the lymphocytes numbers and increase the numbers of capillaries in silica-exposed lung tissues of mice. M. oleifera leaves are potential in preventing diseases.