INVESTIGATING ALPHA AMYLASE ENZYME PRODUCTION BY HUMAN MICROBIOTAYASH SHARMA, GEETIKA SHARMA, JYOTI GUPTA, SHIKHA SINGH, SAHIL KHAN, UTSAV RAJ, ANSHITA NAGAR AND KUMUD BALAAbstract The purpose of the present study was to investigate the amylase enzyme production from human microbiota. Human belly button, ear, nasal and armpits were used as source to observe the microbial load. Biochemical test, molecular characterization (16S rRNA Gene sequencing), phylogentics analysis were used to determine the gram positive and gram negative bacteria of normal flora of human. For screening of amylase enzyme producing human microbiota, starch hydrolysis test, enzyme assay, optimum temperature and optimum pH were performed. Isolates of Enterobacter sp., Bacillus sp. were found to be prominent human microbiota present in human belly button, ear, nasal and armpits that were biochemically and molecularly characterized. Their 16S rRNA gene sequences were submitted to NCBI Gene Bank and accession number were listed down. Only Enterobacter hormaechei (MK503501 & MK503447), Bacillus mycoides (MK503615) and Bacillus subtilis (MK503496) has shown enzyme production and their optimum pH and temperature were studied. 25 oC was found to be optimum temperature of Enterobacter hormaechei (MK503501) and Bacillus mycoides (MK503615), where as Enterobacter hormaechei (MK503447) and Bacillus subtilis (MK503496) has shown 50 oC optimum temperature, respectively. As far as optimum pH wad concerned, specific enzyme activity was found to be present in between pH 5 to 7. Authors would like to suggest using normal flora of human body for the industrial purposes and further to check the pharmaceutical properties like antioxidant and bacteriocin production for future purposes. |