EFFECTIVE USE OF ANTIOXIDANT ACTIVITIES OF FRUIT PEELS OF POMEGRANATE, BANANA AND ORANGE IN BIOREMEDIATION OF CD TOXICITY IN FENUGREEK (TRIGONELLA FOECUM-GRAECUM)AVNEET KAUR, ADITI BHANDRAL, SAKSHI NARULA, SAGRIKA OBEROI, YASHITA JAIN AND NIDHI MITTAL
Fruit peels have various antioxidants and enzyme systems which prevent cellular membranes and organelles from the damaging effects of reactive oxygen species (ROS). Heavy metals, including cadmium (Cd), are associated with oxidative stress, plant damage and change in metabolism pattern in various plants. In the present study, the effect of fruit peel powder of pomegranate, banana and orange at various concentrations of Cd such as 5mM, 10mM, 15mM and 20mM on Glutathione (GSH) content, ascorbic acid level, total phenolic content (TPC), total antioxidant levels were investigated in fenugreek seeds. Fenugreek seeds were grown in heavy metal treated soil (control) and soil with fruit peel powder (pomegranate, banana, orange) and Cd at various concentration (5mM, 10mM, 15mM and 20mM) for 9 days. The present study revealed that there was significant increase (pâ¤0.05) in GSH content, Ascorbic Acid, TPC in plant grown using fruit peel powder to counter the ROS generated by Cd toxicity. There was strong correlation between GSH and ascorbic levels in pomegranate peels (r2=0.927) as compared to orange peels (r2 = 0.4907) and banana peels (r2 = 0.427). The efficiency of fruit peels in scavenging free radicals caused by metal toxicity in increasing order is Pomegranate peels> orange peels > banana peels. Thus, it can be concluded that the fruit peels were able to scavenge free radicals generated by metal toxicity due to their high antioxidant levels, GSH, ascorbic acid, flavonoids and polyphenols levels.
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