Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 1, 2019; Page No.(106-113)

ASSESSMENT OF ACC DEAMINASE CONTAINING ENDOPHYTES ON THE ROOT SYSTEM OF CICER ARIETINUM L. UNDER HYDROPONIC CONDITION

ANNU KUMARIA, KANNEPALLI ANNAPURNA AND AJIT VARMA

Abstract

Endophytic ACC deaminase producing bacteria colonizes the plant tissue and reduces the stress induced damages through suppressing the endogenous level of ethylene hormone.In the present study, endophytic bacteria were isolated from the root nodule of soybean and screened for their ability to use ACC as sole nitrogen source and other PGP activities. Based on the utilization of ACC and in vitro PGP activity, four ACC deaminase containing endophytic bacterial isolates, Enterobacter sp. KA35, Enterobacter cloacae KA1, Pseudomonas stutzeri strain KA30 and Pseudomonas sp. KA28, were selected and their growth promoting activities were assayed with hydroponically grown chickpea (Cicer arietinum L.). Results show that Enterobacter sp. strain KA35 was found to be the most efficient isolate with maximum ACC deaminase activity (1.02 ± 0.28 μmol of α-ketobutyrate /mg/h) and IAA production (21.01± 2.41 μg mL-1) enhanced shoot and root growth, and root length. A significant difference was observed in the number (up to 3.9 fold) and length (upto 4.1 fold) of lateral roots as compared to uninoculated control. We propose here that the plant growth promoting ability of isolates which produces ACC deaminase may potentially increase the root biomass and root branching and support the growth of chickpea.

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