THE SINGLE NUCLEOTIDE POLYMORPHISMS IN IL17AIS ASSOCIATED WITH PULMONARY TUBERCULOSIS IN BABYLON PROVINCE, IRAQMOHAMMED A. AL-SAADI, IFADKERIMABD AL-SHIBLY AND MUSTAFA JAWAD AL-IMARI
Tuberculosis (TB), a disease caused 2 million death status worldwide annually and a quarter of world population latently TB infected (LTBI) with Mycobacterium tuberculosis (MTB) that usually affects the lungs and leading to severe coughing, fever, and chest pains. The polymorphism of many cytokines decreases immunity against this pathogen, IL-17A polymorphisms associated with increased risk to TB infection. To study the correlation between IL-17A polymorphism in PTB patient, PTB contact and healthy control. A case-control study with 60 PTB patients, which visit the chest and respiratory disease center in Babylon province, compared with 60 control (30 PTB contact subject and 33healthy control subject). All the DNA of study subjects was extracted to amplify IL-17A gene by specific primer designed for the purpose of IL-17A amplicon sequencing to detect SNPs. The demographic characteristics show the male more exposure to infection. the male was more frequent cases (58%) especially in age range (35-44), (65-74); while the female less frequent cases (42%) than males and most cases in the age range (15-24). The sequencing of IL-17A amplicon showed two regions have frequent genotype, the AG genotype of rs2275913 was more frequent in PTB with OR=3.5 and the G allele and GG significantly increase in PTB in both rs2275913 and rs3819025 and A allele and AA genotype was lowest frequent in PTB patient that suggest associated with TB resistant and consider protective factor. Male more exposure to pulmonary tuberculosis according to lifestyle. The AA genotype and A allele low frequency in PTB patient compared with contact and control in both gene site.
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