EFFECT OF DIFFERENT CONCENTRATION OF ALPHA TOCOPHEROL SUPPLEMENTATION ON HUMAN BONE MARROW MESENCHYMAL STEM CELL VIABILITY (IN VITRO STUDY)JEFRY WAHYUDI S., NI PUTU MIRA SUMARTA, GATOT BAYDOWI, PURWATI AND COEN PRAMONO D.
Bone defect can be reconstructed with autogenous bone graft and considered as an ideal for bone graft material. The process of bone regeneration that occurs because of the proliferation and differentiation of human bone mesenchymal stem cell marrow. Alpha tocopherol is a co-factor that can help osteogenesis as micronutrient supplementation. Biocompability of this supplement still needs to be proven. This study is to determine the effect of alpha tocopherol supplementation on human bone marrow mesenchymal stem cell viablity. Experimental studies performed in vitro on human bone marrow mesenchymal stem cell. This study was divided into 2 main groups: alpha tocopherol concentrations group (25 µM/mL, 50 µM/mL, 75 µM/mL) and control group. Each group is analyzed with MTT assay observed living cells. Alpha tocopherol with 25 µM/mL concentration obtained high results (107,86%) than other samples (p>0,05 in all subjects observed). Optimum concentration of Alpha tocopherol is 25 µM/mL.
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