CHARACTERISTIC AND PATOGENICITY OF VIBRIO INDIGENOUS SHRIMP PONDSSRI ANDAYANI , AND ATING YUNIARTI
Vibriosis remains a significant problem in the shrimp farming industry in Indonesia. We had isolated four Vibrio indigenous from the shrimp ponds in Java and Bali. This study was intended to characterize those vibrios including their antibiotics resistance, growth character, production of extracellular enzyme and in vivo pathogenity on shrimp. By morphological, biochemical and molecular studies, JP135 and TB651 were confirmed as Vibrio algynoliticus, BL 120 as Vibrio sp and ST112 as V. vulnificus. All isolate was susceptible to antibiotic Streptomycin, Neomycin, Oxytetracycline, Sulphamethaxone, Ciproflaxin and Gentamycin. Antibiotic resistances were recorded on Penicillin, Amphicillin, Chlorampenicol and Erythromycin. Growth characters that were plotted to Logistic model revealed that TB651 had the highest potential to become virulent. Study on phospholipase, lipase, protease dan haemolysin productions showed that all Vibrio was able to produce those in various categories. In vivo pathogenicity on vannamei revealed that LD50 of the Vibrio ranged from 2.3 x 105 to 6.2 x 106 CFU.g-1. In line with the results of the growth character and production of protease, TB651 was recorded as the most virulent isolate. This study provides an information for control and prevention efforts in the future.
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