Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 3, 2016; Page No.(683-685)

OPTIMIZATION OF LIPASE PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM AN OIL INDUSTRY UNIT

B.N. SHUKLA, N.D. PANDYA AND P.V. DESAI

Abstract

This study aims to isolate different organisms that can be used for the purpose of bioremediation for oil contaminated sites. Total eight different bacterial isolates showing good extracellular lipase activity were isolated on tributyrine agar plates from oil contaminated sites. Out of which BN-1(identified as Pseudomonas aeruginosa) showed maximum lipase activity in the production medium (Basal medium) using olive oil as substrate. Lipase activity was quantified spectrophotometrically at 410 nm. Effect of different substrate’s concentration, pH and temperature was measured on the lipase activity.

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