Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 2, 2016; Page No.(483-486)

DETECTION OF GELE AND FACTORS AFFECTING GELATINASE ACTIVITY OF ENTEROCOCCUS FAECALIS ISOLATES

M. PRASANTH, MEERA RAJU, S. DIVAKAR S. ABIRAMI, H. HAJARAH, K. UMA HARINI, S. SENTHILNATHAN AND C. BENEDICT PAUL

Abstract

Virulence factors of E. faecalis have been found to be involved in the pathogenesis of dental diseases. In this study, prevalence of gelatinase and the effect of factors such as glucose concentration and pH have been investigated. Sixty two per cent of the isolates (n=26) were positive for gelE in 42 E.faecalis isolates which were amplified in PCR. Among the gelE positive isolates, 65% (n=17) of the isolates were from Dental Caries. The results showed increasing activity of gelatinase as the concentration of glucose increased upto 1%. We also observed that the gelatinase retained its activity between pH 6 and 8.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.