Ecology, Environment and Conservation Paper

Vol 22, Issue 2, 2016; Page No.(859-863)

IN VITRO CLONAL PROPAGATION OF CAPE GOOSEBERRY (PHYSALIS PERUVIANA L.)

Priyanka Singh, S.P. Singh, Rajesh Shalitra, Sarvesh Singh and Pankaj Kumar Vishwakarma

Abstract

An experiment was conducted for standardization of micro propagation techniques in cape gooseberry (Physalis peruviana L.) via direct organogenesis and indirect callus formation path way. In this study our objective were to develop an efficient micro propagation protocol for cape gooseberry (Physalis peruviana L) by in vitro culture of nodal, internodal and seed explants. Nodal segments were found superior to intermodal segments. In direct organogenesis nodal and intermodal segments were used as an explant. Nodal segments of 1-2 cm having atleast one axillary bud were cultured on Murashige and Skoog (1962) medium supplemented with BAP, Kinetin and IBA. BAP (1.5mg/L), Kinetin (1.0mg/L) and IBA (0.05mg/L) were judged best for shoot proliferation considering sprouting percent, number of shoots, shoot length and number of leaves. The regenerated shoots were transferred to half strength MS medium fortified with IBA for root induction. IBA (0.05mg/L) resulted in best treatment for days to root initiation, rooting percentage, root length and number of roots. In indirect organogenesis seeds were used as explants. BAP (0.9mg/L) +2,4D (0.9 mg/L) was found best for callus induction and for shoot differentiation from callus BAP(1.5mg/ L), Kinetin (1.0mg/L) and IBA (0.05) was best treatment.

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