Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 16, Issue 3, 2014; Page No.(651-657)

PRODUCTION OF AMYLASES BY MICROBIAL AGENTS USING RAW STARCH SUBSTRATE

M.J. MADHUGIRI AND N. BURADE

Abstract

Amylases are among the most important biocatalyst that carry out novel reaction. Today amylase carries out many applications in industrial microbiology. It is widely used in bakery industry for hydrolysis of complex sugar into simple sugars. Bacillary amylases are also used in detergent to dissolve starch from fabrics. In the present study, amylase production was carried out by microbial species like Aspergillus niger and Bacillus subtilis by using raw starch materials like wheat bran, rice bran, maize and wood pulp. For assay of enzymatic activity, substrates were added to fermentation medium using shake flask fermentation. In the current study, Aspergillus niger shows maximium enzymatic activity in shake flask and lab scale fermentation process i.e. 5.70, 2.95, mg/mL in comparison to Bacillus subtilis giving 5.00, 2.75 mg/mL. The yield was found to be higher by using Aspergillus niger in wheat bran substrate in comparison to other substrates.

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