ASSESMENT OF ANTIMICROBIAL POTENCY OF MACTRA VIOLACEAE CRUDE AND PROTEIN ISOLATESM. ARPUTHA BIBIANA, M.S. MANIKANDAN, P. SELVAMANI AND S. LATHA
The marine source was collected from the coast of Cuddalore, Tamil Nadu and it was extracted with 30% acetic acid solution and Tris Hcl buffer. The crude was centrifuged to precipitate and precipitated with Ammonium Sulphate. The attained precipitate was dialyzed with 3.0 kDa membrane using magnetic stirrer and retentates were collected. The antimicrobial activity assay of crude protein extracts of Marine invertebrate was tested against microorganisms by agar well plate method. The marine invertebrate species Mactra violaceae was found to respond to produce the antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. Further the crude extract was fractionated by RP-HPLC C-18 with the gradient range of 5-95% ACN in 0.1% TFA solution. Eight fractions were achieved and the fraction eight (F8) were responded with good antimicrobial potency against E. coli, S. aureus and P. aeruginosa. On SDS-PAGE analysis dark band was obtained with 14 kDa compared to marker. The crude protein extract of Mactra violaceae was also characterized by FTIR. The crude protein content of Mactra violaceae was estimated by biuret method which gives concentration at 500ìg/mL of sample.
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