Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(401-410)

POLYMERASE CHAIN REACTION FOR THE CONFIRMATION OF ISOLATED LISTERIA SPP. FROM FOOD CONTACT SURFACES IN THE OPEN MARKETS IN KHONKAEN, THAILAND

P. PASUWAN, R. R. BEUMER, H. DEN BESTEN AND B. LEENANON

Abstract

Swab samples (280) were taken from different kinds of food contact surfaces including stainless steel, tile, polyethylene and wooden cutting board from five open markets in KhonKaen, Thailand. Listeria spp. was found from food contact surfaces in three open markets. Most positive samples were isolated from surfaces of stainless steel, wooden cutting board, ceramic tile and polyethylene. All suspected colonies were identified using biological tests such as Gram stain, mobility, catalase, and CAMP Tests. The weak hemolytic colonies of the CAMP reaction on 5% sheep blood agar defined that 13 out of 70 colonies were identified as L. monocytogenes. Listeria identification tests were performed by 10 Listeria API strips. All weak hemolytic strains were confirmed by PCR. PCR products amplified with 16sRNA primers were sent for sequencing. Results showed that all isolated strains amplified with the general Listeria primer were confirmed as Listeria spp. However, the PCR products bands which were amplified specifically for L. monocytogenes were missing on 1.5% Agarose gel. The PCR results implied that all isolated strains were not L. monocytogenes. Blasting results from EMBL-EBI and Ezbiocloud databases reported that all isolated strains with weak hemolytic results were L. innocua.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 23, Issue 1, 2021
Vol 22, Issue 4, 2020
Vol 22, Issue 3, 2020
Vol 22, Issue 2, 2020
Vol 22, Issue 1, 2020
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!